Eukaryotic RNA Processing

Professor Miesfeld at the University of Arizona

This Online Biochemistry Course is based on Miesfeld’s Biochemistry (3rd Edition)

Primary transcripts are processed into mature mRNA, tRNA, and rRNA using ribozyme chemistry and the spliceosome. This video covers self-splicing introns, snRNP assembly, the two steps of lariat formation, alternative splicing, and splicing defects that cause disease such as retinitis pigmentosa.

Key concepts covered: RNA processing, ribozymes and the RNA world, hammerhead ribozyme, group I and group II self-splicing introns, spliceosome and snRNPs, 5′ GU / branch-point A / 3′ AG splice sites, transesterification, lariat formation, alternative splicing, retinitis pigmentosa.