Regulation of Metabolic Flux in Glycolysis

Professor Miesfeld at the University of Arizona

This Online Biochemistry Course is based on Miesfeld’s Biochemistry (3rd Edition)

Glycolysis is controlled at its three irreversible steps: hexokinase or glucokinase, phosphofructokinase-1, and pyruvate kinase. This video covers glucokinase as a glucose sensor (including GKRP and insulin release in pancreatic beta cells) and the allosteric control of PFK-1 and pyruvate kinase.

Key concepts covered: metabolic flux control, irreversible glycolytic steps, hexokinase vs. glucokinase Km, glucokinase regulatory protein (GKRP), glucose sensing and insulin release, allosteric regulation of PFK-1 (ATP, AMP, fructose-2,6-bisphosphate, citrate), feed-forward activation of pyruvate kinase.