Regulation of O2 Binding to Hemoglobin

Professor Miesfeld at the University of Arizona

This Online Biochemistry Course is based on Miesfeld’s Biochemistry (3rd Edition)

Miesfeld BIOC 384 Module 4: Topic 6 – Regulation of O2 Binding to Hemoglobin

Bioc384-B3e_M04.T06-HemoglobinAllostery.RLM Download

Oxygen, CO2, H+, and 2,3-BPG act as allosteric regulators that switch hemoglobin between its T and R states to match oxygen delivery to what tissues need. This video covers the Bohr effect, carbamate formation, 2,3-BPG binding, and why fetal hemoglobin binds oxygen more tightly than adult hemoglobin.

Key concepts covered: allosteric regulation, T-state and R-state hemoglobin, the Bohr effect, His146 and Asp94, CO2 transport, carbonic anhydrase, carbamate formation, 2,3-BPG, fetal vs. adult hemoglobin, oxygen saturation curves.